Time Schedule:
Scott S. Graves
MED 499
Seattle Campus
Case studies, with laboratory research. Available to undergraduates and medical students.
Class description
The overal goal is to insturct a student in the various aspects of functioning as a laboratory technician inthe field of immunolgy. The class is located at the Fred Hutchinson Cancer Research Center. Specifically, the student will be expected to learn the concepts of designing and building an engineered fusion protein. Based on the intensity of the work and laboratory size, only 1 student with the appropriate background can be selected per quarter on a first come basis.
Student learning goals
Molecular biology techniques including: PCR, restriction enzymatic digestion, gel electrophoresis, primer design, cloning.
ELISA techniques
concepts of immune tolerance and costimulatory molecule blockade
tissue culture
General method of instruction
The student will participate in an active lab environment under the direction of a Lab director and lab technician. Background and instruction will be supplied using basic manuals and recent manuscripts.
Recommended preparation
Cell biology, biochemistry.
Class assignments and grading
The student will be given an assignment of constructing an engineered protein. The student will be graded on attendance, attention to detail, lab notebook clarity, level of completion of the project, and oral presentation.
The student will be required to write-up the results of the assignment including: introduction with hypothesis, methods, results and discussion sections. Additionally, the student will be required to give an oral presentation of the work in a general laboratory meeting.
