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Instructor Class Description

Time Schedule:

Scott S. Graves
MED 499
Seattle Campus

Undergraduate Research

Case studies, with laboratory research. Available to undergraduates and medical students.

Class description

The overal goal is to insturct a student in the various aspects of functioning as a laboratory technician inthe field of immunolgy. The class is located at the Fred Hutchinson Cancer Research Center. Specifically, the student will be expected to learn the concepts of designing and building an engineered fusion protein. Based on the intensity of the work and laboratory size, only 1 student with the appropriate background can be selected per quarter on a first come basis.

Student learning goals

Molecular biology techniques including: PCR, restriction enzymatic digestion, gel electrophoresis, primer design, cloning.

ELISA techniques

concepts of immune tolerance and costimulatory molecule blockade

tissue culture

General method of instruction

The student will participate in an active lab environment under the direction of a Lab director and lab technician. Background and instruction will be supplied using basic manuals and recent manuscripts.

Recommended preparation

Cell biology, biochemistry.

Class assignments and grading

The student will be given an assignment of constructing an engineered protein. The student will be graded on attendance, attention to detail, lab notebook clarity, level of completion of the project, and oral presentation.

The student will be required to write-up the results of the assignment including: introduction with hypothesis, methods, results and discussion sections. Additionally, the student will be required to give an oral presentation of the work in a general laboratory meeting.

The information above is intended to be helpful in choosing courses. Because the instructor may further develop his/her plans for this course, its characteristics are subject to change without notice. In most cases, the official course syllabus will be distributed on the first day of class.
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Last Update by Scott S. Graves
Date: 12/12/2012