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Instructor Class Description

Time Schedule:

Dale Whittington
Seattle Campus

Biological Mass Spectrometry

Covers the basics of modern ionization methods and mass analyzers; small molecule structure assignment, quantitative assay development by LC-MS and metabolomics; quantitative discovery-based proteomics and validation methods; and peptide sequence determination, post-translational modification mapping, and protein structure determination methods. Prerequisite: permission of instructor. Offered: Sp.

Class description

Basic principles of mass spectrometry and its application to the research and clinical setting. Includes: TOF, MALDI, MS/MS principles, spectral interpretation, quantitation & validation of analytical assays.

Student learning goals

Describe the differences between classes of Mass Spectrometers technology.

Explain how different types of Mass Spectrometers obtain data.

Interpret Mass Spectra for structure elucidation.

Build analytical curves for quantitative purposes.

General method of instruction

Lectures and hands on application

Recommended preparation

Class assignments and grading

Course grading will be based on scores obtained on 3 take-home exams (25% each) and one lab practical (25%).

The lab practical is worth 100 points (25% of your final grade). The points will be awarded as follows: In written form a description of preparation of samples (25), description of the system used for analysis (25), write up of how the setting chosen for analysis were determined (25), and an analysis page of the results of the standard curve, QC’s and unknown samples (25).

The information above is intended to be helpful in choosing courses. Because the instructor may further develop his/her plans for this course, its characteristics are subject to change without notice. In most cases, the official course syllabus will be distributed on the first day of class.
Last Update by Dale Whittington
Date: 03/20/2013