Covers the basics of modern ionization methods and mass analyzers; small molecule structure assignment, quantitative assay development by LC-MS and metabolomics; quantitative discovery-based proteomics and validation methods; and peptide sequence determination, post-translational modification mapping, and protein structure determination methods. Prerequisite: permission of instructor. Offered: Sp.
Basic principles of mass spectrometry and its application to the research and clinical setting. Includes: TOF, MALDI, MS/MS principles, spectral interpretation, quantitation & validation of analytical assays.
Student learning goals
Describe the differences between classes of Mass Spectrometers technology.
Explain how different types of Mass Spectrometers obtain data.
Interpret Mass Spectra for structure elucidation.
Build analytical curves for quantitative purposes.
General method of instruction
Lectures and hands on application
Class assignments and grading
Course grading will be based on scores obtained on 3 take-home exams (25% each) and one lab practical (25%).
The lab practical is worth 100 points (25% of your final grade). The points will be awarded as follows: In written form a description of preparation of samples (25), description of the system used for analysis (25), write up of how the setting chosen for analysis were determined (25), and an analysis page of the results of the standard curve, QC’s and unknown samples (25).