Time Schedule:
Richard Y To
BIOEN 599
Seattle Campus
Offered at a graduate level periodically by faculty members within the Department of Bioengineering; concerns areas of research activities with current and topical interest to bioengineers. Prerequisite: undergraduate or graduate courses (or equivalent) determined individually for each special topic.
Class description
This course is designed to introduce practical basic recombinant DNA techniques to engineers and other non-biologists who would like to gain hands-on experiences in biotechnology. Laboratory projects include DNA extraction and purification, site-directed mutagenesis, DNA ligation and sequencing. Other techniques include Polymerase Chain Reaction (PCR), protein expression, DNA and protein gel electrophoresis (including Western transfer), and immunosorbent assays (ELISA). Cell culture techniques include bacteria transformation and mammalian cell transfection.
Student learning goals
Basic DNA cloning techniques: DNA sequencing, PCR, restriction analysis, ligation.
DNA site directed mutagenesis
Protein expression: SDS PAGE, ELISA
Basic mammalian cell culture and transfection
Bacterial culture and transformation
General method of instruction
Lecture Laboratory project
Recommended preparation
General Chemistry required Biochemistry preferred Basic biology preferred
Class assignments and grading
Laboratory notebook 60% 4 homeworks 4 X 5% = 20% Final exam 20%
