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Instructor Class Description

Time Schedule:

Richard Y To
BIOEN 599
Seattle Campus

Special Topics in Bioengineering

Offered at a graduate level periodically by faculty members within the Department of Bioengineering; concerns areas of research activities with current and topical interest to bioengineers. Prerequisite: undergraduate or graduate courses (or equivalent) determined individually for each special topic.

Class description

This course is designed to introduce practical basic recombinant DNA techniques to engineers and other non-biologists who would like to gain hands-on experiences in biotechnology. Laboratory projects include DNA extraction and purification, site-directed mutagenesis, DNA ligation and sequencing. Other techniques include Polymerase Chain Reaction (PCR), protein expression, DNA and protein gel electrophoresis (including Western transfer), and immunosorbent assays (ELISA). Cell culture techniques include bacteria transformation and mammalian cell transfection.

Student learning goals

Basic DNA cloning techniques: DNA sequencing, PCR, restriction analysis, ligation.

DNA site directed mutagenesis

Protein expression: SDS PAGE, ELISA

Basic mammalian cell culture and transfection

Bacterial culture and transformation

General method of instruction

Lecture Laboratory project

Recommended preparation

General Chemistry required Biochemistry preferred Basic biology preferred

Class assignments and grading

Laboratory notebook 60% 4 homeworks 4 X 5% = 20% Final exam 20%


The information above is intended to be helpful in choosing courses. Because the instructor may further develop his/her plans for this course, its characteristics are subject to change without notice. In most cases, the official course syllabus will be distributed on the first day of class.
Last Update by Richard Y To
Date: 09/28/2010