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Instructor Class Description

Time Schedule:

Dominic W Chung
BIOC 426
Seattle Campus

Basic Techniques in Biochemistry

Introduction to basic biochemistry experiments. Acquaints students (largely biochemistry majors) with basic biochemical laboratory techniques. Prerequisite: BIOC 440, which may be taken concurrently. Offered: ASp.

Class description

Basic techniques commonly used in biochemical studies, including affinity chromatography, polyacrylamide and agarose gel electrophoresis, enzyme kinetics, ELISA, Western blot, chemical modification of proteins, polymerase chain reaction, molecular cloning, and DNA sequencing.

Student learning goals

Learn the theory and concepts behind the techniques.

Analyze and interpret results.

Deduce and draw conclusions.

General method of instruction

Hands-on experience, and working in teams, with instructor demonstrations.

Recommended preparation

Concurrent enrollment in or completion of the Bioc 440, 441, 442 lecture series. Simple statistical analyses of experimental data, and use of computer programs such as Word, Excel and PowerPoint.

Class assignments and grading

1. Short homework assignments, emphasizing preparation before class. 2. Observations during class, emphasizing accuracy and thoroughness. 3. Quizzes, emphasizing overall understanding of concepts. 4. Laboratory reports, emphasizing analytical techniques, reasoning, clarity of written communication.

The class is graded on a curve based on points accumulated from homework assignments, three written exams and three lab reports (no final exam). Typical class average grade is 3.2.


The information above is intended to be helpful in choosing courses. Because the instructor may further develop his/her plans for this course, its characteristics are subject to change without notice. In most cases, the official course syllabus will be distributed on the first day of class.
Additional Information
Last Update by Dominic W Chung
Date: 03/29/2010