Dominic W Chung
Introduction to basic biochemistry experiments. Acquaints students (largely biochemistry majors) with basic biochemical laboratory techniques. Prerequisite: BIOC 440, which may be taken concurrently. Offered: ASp.
Basic techniques commonly used in biochemical studies, including affinity chromatography, polyacrylamide and agarose gel electrophoresis, enzyme kinetics, ELISA, Western blot, chemical modification of proteins, polymerase chain reaction, molecular cloning, and DNA sequencing.
Student learning goals
Learn the theory and concepts behind the techniques.
Analyze and interpret results.
Deduce and draw conclusions.
General method of instruction
Hands-on experience, and working in teams, with instructor demonstrations.
Concurrent enrollment in or completion of the Bioc 440, 441, 442 lecture series. Simple statistical analyses of experimental data, and use of computer programs such as Word, Excel and PowerPoint.
Class assignments and grading
1. Short homework assignments, emphasizing preparation before class. 2. Observations during class, emphasizing accuracy and thoroughness. 3. Quizzes, emphasizing overall understanding of concepts. 4. Laboratory reports, emphasizing analytical techniques, reasoning, clarity of written communication.
The class is graded on a curve based on points accumulated from homework assignments, three written exams and three lab reports (no final exam). Typical class average grade is 3.2.