UW Research

Genome Sciences Pacific Biosystems Facility

VISIT CENTER

Description

The single molecule real-time (SMRT) sequencing is a third generation sequencing that allows for long-read sequencing. With the RSII technology, we are able to get an average of 4-5 Kb of sequence read with 3-20 Kb library preparations, generating about 400 Mb per SMRT cell. We are an HHMI-designated Pacific Biosciences (PacBio) sequencing site offering services for library preparation as well as sample sequencing.

  • We provide library preparations and sequencing for:
    – Large-insert gDNA (3-20 Kb)
    – BAC-based DNA
    – Purified PCR products
    – Whole genome sequencing small genomes

Type

Equipment: RSII Sequencer

Keywords: General

Analytical/bioanalytical, Spectroscopy, Analytical Chemistry, Laboratory
Chemical Analysis, AIHA-LAP, LLC accredited, ISO/IEC 17025 Compliant

Keywords: Specific

EPA methods, NIOSH methods, OSHA methods, Breathing Air analysis, Organophosphate metabolite assay, PAH assay, Phthalate metabolite, Long read sequencing, Mass spectrometry, Mass spectrometry assay, Metabolite assay, High-performance liquid chromatography (HPLC),
FT-IR spectrometer, Gas chromatography, Gas chromatography-mass spectrometry, GC-MS assay,FTIR, GCFID, GC-FID, GCMS, GC-MS, GCMSMS, GC-MS-MS, HPLC, IC, ICPMS, LCMSMS, LC-MS-MS