Do you have an example of what type of information I should provide in the “Abstract”?
Below is an excerpt of an ideal Abstract description:
Abstract (describe in 250 words or less, in layman’s terms, the goals of the research, rationale for using hESC lines, brief description of the approach, and benefits to society)
Goals: To assess the efficacy of newly generated hESC lines by investigating the fate of hES cells that were 1) injected into various sites within the adult mouse, to test their ability to generate teratomas, e.g., sub-cutaneous, intramuscular, kidney capsules and testes, or 2) injected into a mouse embryo to generate xeno-chimeras, to test their normal development up to mouse embryonic day 11.5.
Rationale & Benefit: Teratoma formation is the only assay we currently have to assess the array of tissues that can be formed from a particular hESC line. Given that these tissues are tumors, growth is disorganized and difficult to relate to normal development. Contribution to chimeras provides a context to the development. If a cell is present within a particular tissue, development is considered normal and the starting hESC lines would be thought to have the capability of forming that tissue. hESC lines have different abilities following directed in vitro differentiation. There are as many protocols for directed differentiation as there are investigators and target tissues. Development within the in vivo context of a chimera could potentially allow us to assess the ability of any particular line more accurately than either in vitro assays or teratoma formation. We will start this project using non-human primate ES cell lines, with the plan that if the primate-cell assays work well, and once ESCRO approval is given, we will proceed to assess all human ES cell lines in this fashion.
Methods: Direct injection of undifferentiated and differentiated hESCs and iPSCs into tissues in question.