What if I want to use derivatives from hESC lines or create genetically different cells from hESC lines (e.g. cardiomyocytes)?
Yes, if the hESC-derived cell line still retains the ability to create all three embryonic germ layers. According to section V.D. of GIM 36, “Except as provided in section V. E. below, all hESC research, including research involving the use or creation of a hESC-derived cell line, must be reviewed and approved by the ESCRO Committee prior to the commencement of the research.” An hESC-derived line means, “one or more cells or cell line, including a hESC line created from the destruction of a human embryo, but does not include a hESC line created by SCNT without destruction of a human embryo.”
For example, modifying the H7 cell line by addition of a GFP-reporter would create a genetically distinct hESC-derived cell line that still retains the characteristics of an embryonic stem cell; use of this new cell line would require ESCRO approval.
No, if the hESC derivative no longer retains the ability to generate all three embryonic germ layers. A hESC derivative is defined as “any DNA, RNA, protein, or other biological products secreted by or extracted from a hESC, including an adult stem cell, but does not include any HESC line, HESC-derived cell line, data, or intellectual property.”