Instructor Class Description

Time Schedule:

• Summer Quarter 2008
• Autumn Quarter 2008

Daniel Bowen-Pope
PATH 510
Seattle Campus

Introduction to Pathology Methods

Laboratory course designed to introduce graduate students to the fundamentals of image analysis, histology, histopathology, post mortem evaluation, surgical pathology, and other methods used to investigate disease etiology, progression, and manifestation in humans and in animal models. Prerequisite: permission of instructor. Offered: Sp.

Class description

Summary of course content: This is a laboratory course designed to introduce graduate students to the fundamentals of image analysis, histology, histopathology, post mortem evaluation, surgical pathology, and other methods used to investigate disease etiology, progression, and manifestation in humans and in animal models.

Histology and histopathology sessions: The goal is to teach the basics of histology and histopathology. This segment will include:

Introduction to basic histology techniques. Lecture and hands-on for fixation, paraffin embedding, microtome sectioning, H&E staining. Lecture and demo of microscope set up and use. Demo and discussion of methodology and critical interpretation of immunohistochemistry and in situ hybridization.

Approximately 7 sessions on microscope with a digital display. Practicing pathologists in our department will provide a practical introduction to the recognition and interpretation of normal and pathological tissue structure.

Cytogenetics [Swisshelm] Lecture/demo

Clinical Molecular Genetics [Karen Stephens] Lecture/demo

Autopsy sessions: Directed by Corinne Fligner, M.D., Director of Autopsy Service Corinne Fligner 1) Introduction Dr. Fligner has a 45min videotape on the autopsy that she shows to med students before they attend an autopsy. We will watch it then meet with her for a question and answer session. She will also tell us what will be worn, what to expect, etc.

2) Attending the autopsy. I will inform you by email at 8:30 on Monday morning if there will be an autopsy. If so, go down. Can take 2-3hrs.

3) Following wednesday: The removed fixed organs will be examined grossly, and often additional samples for sectioning will be taken. Takes about 1hr and may include tissues from 2-5 autopsies

Surgical pathology. [Approx 4hrs] Section Leader: Larry True, M.D. 8-4027 ltrue@u.washington.edu

lecture session: Surgical prostate tissue good example of a tissue allocation program. Feeds into the Hood program to identify genes involved in prostatic carcinoma. Tissue frozen for RNA extraction. At this didactic session Larry should use kodachromes to introduce normal and pathologies of prostate, explain how Hood’s gene identification program works, ...

Demo session: Urology has first priority for the OR on Mondays so most nonemergency urology surgery is done mon. Number of Monday prostatectomies ranges from 0-3 with an average of 1. The number for a Monday is known by the previous Friday. The time that the tissue arrives is not as predictable. Generally 10:00 for arrival of the lymph nodes [95% of these turn up negative].

Margins are inked. About 3 thick [several mm] coronal slices are taken and frozen. From these, cryostat sections are cut, H&E stained [takes minutes] and evaluated to inform the surgeon re completeness of tumor resection. Larry usually looks at the stained sections within 1 hr, say at about 1:00 The cryostat sections then guide the dissection of tumor tissue from the thick sections. This is placed in lysis buffer.

Flow cytometry session [Rabinovitch/Poot]. Introduction and demonstration of the use of flow cytometry for evaluation of experimental and surgical specimens.

Using animals in research [Buetow]. Introduction to the rules and regulations and “behind the scenes” exploration of the UW animal facilities and procedures.

Rodent models of disease [Murry]. Laboratory and discussion session demonstrating a rodent models of cardic injury/response to illustrate how cardiovascular pathologies can be modelled in animals.

Research at a biotech company (ZymoGenetics): Database mining [David Adler] Protein purification, production and testing and marketing [Charlie Hart]

Student learning goals

General method of instruction

Small group hands/eyes-on sessions

Recommended preparation

Class assignments and grading

Read assigned sections on histology and histopathology

Attendance and participation

Office of the Registrar
For problems and questions about this web page contact icd@u.washington.edu,
otherwise contact the instructor or department directly.
Modified:August 20, 2008