Contact Information

University of Washington School of Medicine
NW145, Box 356069
1959 N.E. Pacific Street
Seattle, WA 98195
Tel: 206-598-4094 (Office)
Tel: 206-598-6813 (Lab)
Fax: 206-598-6813
E-mail: ygu@u.washington.edu

Education

6/84 B.S. in Biochemistry, East China University of Chemical Technology
Thesis title: Topology of the subunits of QH2:cytochrome c reductase from chicken heart---photolabeling of the hydrophobic domain
Advisor: Yun Li, Ph.D.

12/93 Ph.D. in Genetics, Thomas Jefferson University
Thesis title: Molecular genetics of human acute leukemia with t(4;11)(q21;q23)
Advisor: Carlo M. Croce, M.D.

Postdoctoral training: 1/94-4/94 Cancer genetics
Kimmel Cancer Institute, Thomas Jefferson University
Advisors: Carlo M. Croce, M.D. & Eli Canaani, Ph.D.

4/94-2/01 Molecular immunology
Harvard Medical School
Advisor: Frederick W Alt, Ph.D.

Professional positions

84-87 Research Assistant, Shanghai Center for Clinical Laboratory, China

87-89 Research Assistant, Wistar Institute, Philadelphia

94 Postdoctoral Fellow, Kimmel Cancer Institute, Philadelphia

94-01 Research Fellow, Department of Genetics, Harvard Medical School

97-00 Associate, Howard Hughes Medical Institute, Children's Hospital

01-present Assistant Professor, Department of Radiation Oncology, UW

01-present Adjunct Assistant Professor, Department of Immunology, UW

Awards and Honors

4/94 Cancer Research Institute Fellow

Research Funding

Agency: Nathan Shock Center of Excellence in the Basic Biology of Aging
Title: Mouse Models for Study of SIR2 Function in Development and Aging
Period: 7/1/01-6/30/02 ($30,000)
Role: PI

Agency: NIH (RO1 PI: Nelson Fausto)
Title: Pathogenesis of Hepatocellular Carcinomas
Period: 4/1/02-3/31/07 ($200,000 per year)
Role: Collaborator (5%)

Teaching

Cancer Biology for Residents (organize, and teach 11 of 23 lectures in Winter and Spring, 2002)
R ONC 499 Undergraduate Research

Service

Consultant: UW Mouse Genomic Center (Director, Warren Ladiges)(01-present)
Reviewer: UW Royalty Research Fund (Spring and Fall, 2001)

Publications

1. Li Y, Gu Y & Lin CS. (1986). Topology of the subunits of QH2:cytochrome c reductase from chicken heart--photolabeling of the hydrophobic domain. Acta Biochemica et Biophysica Sinica 18(1), 27-34.

2. Cimino G, Nakamura T, Gu Y, Canaani O, Prasad R, Crist W, Carrol A, Baer M, Bloomfield CD, Nowell PC, Croce CM & Canaani E. (1992). Cloning of ALL-1, the locus involved in leukemias with the t(4:11)(q21:q23), t(9:11)(p22:q23) and t(11:19)(q23:p13) chromosome translocation. Cancer Research 52, 3811-3813.

3. Gu Y, Cimino G, Alder H, Nakamura T, Prasad R, Canaani O, Moir DT, Jones C, Nowell PC, Croce CM & Canaani E. (1992). The (4:11)(q21:q23) chromosome translocations in acute leukemia involve the VDJ recombinase. Proc. Natl. Acad. Sci. USA 89, 10464-10468.

4. Gu Y, Nakamura T, Alder H, Prasad R, Canaani O, Cimino G, Croce CM & Canaani E. (1992). The t(4:11) chromosome translocation of human acute leukemias fuses the ALL-1 gene, related to Drosophila trithorax, to the AF-4 gene. Cell 71, 701-708.

5. Nakamura T, Alder H, Gu Y, Prasad R, Canaani O, Kamada N, Gale RP, Lange B, Crist WM, Nowell PC, Croce CM & Canaani E. (1993). Genes on chromosomes 4, 9, and 19 involved in 11q23 abnormalities in acute leukemia share sequence homology and/or common motifs. Proc. Natl. Acad. Sci. USA 90, 4631-4635.

6. Ma Q, Alder H, Nelson KK, Chatterjee D, Gu Y, Nakamura T, Canaani E, Croce CM, Siracusa LD & Buchberg AM. (1993). Analysis of the murine All-1 gene reveals conserved domains with human ALL-1 and identifies a motif shared with DNA methyltransferases. Proc. Natl. Acad. Sci. USA 90, 6350-6354.

7. Prasad R, Gu Y, Alder H, Nakamura T, Canaani O, Saito H, Huebner K, Gale RP, Nowell PC, Kuriyama Y, Miyazaki Y, Croce CM & Canaani E. (1993). Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in AMLs with the t(6;11) chromosome translocation. Cancer Research 53, 5581-5585.

8. Gu Y, Alder H, Nakamura T, Schichman SA, Prasad R, Canaani O, Saito H, Croce CM & Canaani E. (1994). Sequence analysis of the breakpoint cluster region in the ALL-1 gene involved in acute leukemia. Cancer Reserach 54, 2327-2330.

9. Schichman SA, Caligiuri MA, Gu Y, Strout MP, Canaani E, Bloomfield CD & Croce CM. (1994). ALL-1 partial duplication in acute leukemia. Proc. Natl. Acad. Sci. USA 91, 6236-6239.

10. Prasad R, Leshkowitz D, Gu Y, Alder H, Nakamura T, Saito H, Huebner K, Berger R, Croce CM & Canaani E. (1994). Leucine-zipper dimerization motif encoded by the AF-17 gene fused to ALL-1 (MLL-1) in acute leukemia. Proc. Natl. Acad. Sci. USA 91, 8107-8111.

11. Schichman SA, Caligiuri MA, Strout MP, Carter SL, Gu Y, Canaani E, Bloomfield CD & Croce CM. (1994). ALL-1 tandem duplication in acute myeloid leukemia with a normal karyotype involves homologous recombination between Alu elements. Cancer Research 54, 4277-4280.

12. Prasad R, Yano T, Sorio C, Nakamura T, Rallapalli R, Gu Y, Leshkowitz D, Croce CM & Canaani E. (1995). Domains with transcriptional regulatory activity within the ALL1 and AF4 proteins involved in acute leukemia. Proc. Natl. Acad. Sci. USA 92,12160-12164.

13. Druck T, Gu Y, Prabhala G, Cannizzaro LA, Park S-H, Huebner K & Keen JH (1995). Chromosome localization of human genes for clathrin adaptor polypeptides AP2b and AP50 and the clathrin-binding protein, VCP. Genomics 30, 94-97.

14. Gu Y, Jin S, Gao Y, Weaver DT & Alt FW. (1997). Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination. Proc. Natl. Acad. Sci. U.S.A. 94,8076-8081.

15. Gu Y, Seidl KJ, Rathbun GA, Zhu C, Manis JP, van der Stoep N, Davidson L, Cheng H-L, Sekiguchi JM, Frank K, Stanhope-Baker P, Schlissel MS, Roth DB & Alt FW. (1997). Growth retardation and leaky SCID phenotype of Ku70-deficient mice. Immunity 7, 653-665.

16. Manis JP, Gu Y, Lansford R, Ferrini R, Davidson L, Rajewsky K & Alt FW. (1998). Ku70 is required for late B cell development: a potential role in Ig heavy chain class switching. J. Exp. Med. 187,2081-2089.

17. Alt FW, Frank K, Sekiguchi J, Gao Y, Gu Y, Chaudhuri J, Rathbun G, Seidl KJ, Weaver D. (1998). General-expressed gene products involved in VDJ recombination and DNA repair. Molecular Immunology 35: (11-12) 681-682.

18. Sekiguchi JM, Gao Y, Gu Y, Frank K, Sun Y, Chaudhuri J, Zhu C, Cheng HL, Manis J, Ferguson D, Davidson L, Greenberg ME, Alt FW. (1999). Nonhomologous end-joining proteins are required for V(D)J recombination, normal growth, and neurogenesis. Cold Spring Habor Symposia on Quantitative Biology 64: 169-181.

19. Gu Y, Sekihuchi JM, Gao Y, Dikkes P, Frank K, Ferguson DO, Hasty P, Chun J & Alt FW. (2000). Defective embryonic neurogenesis in Ku, but not DNA-PKcs, deficient mice. Proc. Natl. Acad. Sci. U.S.A. 97, 2668-2973.

20. Borghesani PR, Alt FW, Bottaro A, Davidson L, Aksoy S., Rathbun GA, Roberts TM, Swat W, Segal RA & Gu Y (2000). Abnormal development of Purkinje cells and lymphocytes in Atm mutant mice. Proc. Natl. Acad. Sci. U.S.A. 97, 3336-3341.

21. Sekiguchi JM, Ferguson DO, Chen H-T, Yang EM, Earle J, Frank K, Whitlow S, Gu Y, Xu Y, Nussenzweig A, & Alt FW. (2001). Genetic interaction between Atm and the non-homologous end-joining factors in genomic stability and development. Proc. Natl. Acad. Sci. U.S.A. 98, 3243-3248.

22. Ferguson DO, Sekiguchi JM, Frank K, Gao Y, Sharpless NE, Gu Y, Manis J, DePinho RA & Alt FW (2001). The interplay between non homologous end joining and cell cycle checkpoint factors in development, genomic stability and tumorigenesis. Cold Spring Habor Symposia on Quantitative Biology In press.

Students (research projects only)

Yreka Ocamp, UW '02, Cellular and Molecular Biology 4/01-present

Michael Lee, UW '03, Neurobiology 6/01-present

Laura Eng, UCLA '03, Molecular and Cellular Biology 6-9/01

Christina Khodr, Trinity College '02, Neurobiology 6-9/01